Phosphatase Activity in Porcine Brain Microtubule Proteins
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چکیده
منابع مشابه
Brain tubulin polymerization in the absence of "microtubule-associated proteins".
Pure tubulin dimer, purified by phosphecellulose chromatography to remove microtubule associated proteins, can be assembled into microtubules in the presence of dimethyl sulfoxide. The surfaces of such microtubules are devoid of filamentous material. The reaction is rapid and strongly dependent on protein concentration. No microtubule formation occurs from the purified tubulin when dimethyl sul...
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An ATPase activity is persistently associated with bovine brain microtubules even after multiple cycles of warm-induced microtubule assembly, centrifugation through a 50% sucrose cushion, cold-induced disassembly, and subsequent centrifugation. Likewise, a sucrose extraction method for preparing microtubule proteins free of mitochondrial contamination does not eliminate ATPase activity in th...
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Protein phosphatase 2A (PP2A) is one of the most abundant serine-threonine phosphatases in mammalian cells. PP2A is a hetero-trimeric holoenzyme participating in a variety of physiological processes whose deregulation is often associated to cancer. The specificity and activity of this phosphatase is tightly modulated by a family of regulatory B subunits that dock the catalytic subunit to the su...
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ژورنال
عنوان ژورنال: YAKUGAKU ZASSHI
سال: 1985
ISSN: 0031-6903,1347-5231
DOI: 10.1248/yakushi1947.105.5_464